Luminex 100 IS Developer Workbench Guide Version 2.3 Manual de usuario Pagina 26
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Luminex
100 IS Developer Workbench Guide Version 2.3 xMAP Technology
20 PN 89-00002-00-084 Rev. B
17. These commands become part of the template and are always
performed in the order specified. Each well command is
automatically assigned to the next available well. Laboratory
personnel can select the starting location on the plate, but they
can not change these commands or the order in which they are
performed. Remember that if you have more than one command
that involves the reservoir, those commands must use the same
solution.
Table 3 lists available template commands.
18. After you enter all the information, click Save to save the
template, then click Close to exit.
Table 3: Available Commands
Command Definition
Acquire Acquires an unknown sample.
Acquire
Background
Acquires a background sample from a well. The
background results are automatically subtracted
from standards, controls, and samples. If you are
running more than one background well, the
software will subtract the average of the
background samples.
Acquire Control Acquires the specified control from a well. If a
specified control is selected more than once, the
software calculates the results for each well
independently. It then reports an average of the
control samples.
Acquire Standard Acquires the specified standard from a well. If a
specified standard is selected more than once, the
software calculates the results for each well
independently. It then reports an average of the
two samples.
Acquire Test
Specimen
Acquires a sample reading from a well. If Acquire
Test Specimen is selected more than once, the
software calculates the results for each well
independently. However, samples having identical
Sample IDs are treated as replicates reporting an
average of the individual samples.
Backflush Backflushes the system by reversing the flow of
sheath fluid through the cuvette.
Calibrate CAL1 Acquires calibration beads from a well to calibrate
the Classification and DD channels.
Calibrate CAL2 Acquires calibration beads from a well to calibrate
the Reporter channel.
Note: You can return and edit
any unused templates using the
Edit Template feature.
- Luminex 100 1
- IS Developer 1
- Workbench Guide Version 2.3 1
- PN 89-00002-00-084 Rev. B 2
- August, 2005 2
- Contents 3
- A xMAP Protocols 49 4
- Version 1.7 61 5
- C Customize Splash Screen 63 5
- Index 65 5
- Technology 6
- Developer Workbench Guide 7
- General Guidelines 8
- Instructions 9
- Instrument Calibrators 12
- Developer 13
- Workbench 13
- Software 13
- Procedures 14
- Dialog Box Buttons 15
- Create New Product 15
- 10 PN 89-00002-00-084 Rev. B 16
- PN 89-00002-00-084 Rev. B 11 17
- 12 PN 89-00002-00-084 Rev. B 18
- PN 89-00002-00-084 Rev. B 13 19
- 14 PN 89-00002-00-084 Rev. B 20
- Quantitative Assay 21
- Template 21
- 16 PN 89-00002-00-084 Rev. B 22
- PN 89-00002-00-084 Rev. B 17 23
- Note: You can use the same 23
- 18 PN 89-00002-00-084 Rev. B 24
- Figure 7. Select Bead IDs 24
- PN 89-00002-00-084 Rev. B 19 25
- Qualitative Assay 27
- 22 PN 89-00002-00-084 Rev. B 28
- PN 89-00002-00-084 Rev. B 23 29
- 24 PN 89-00002-00-084 Rev. B 30
- PN 89-00002-00-084 Rev. B 25 31
- Figure 13. Select Bead IDs 31
- 26 PN 89-00002-00-084 Rev. B 32
- PN 89-00002-00-084 Rev. B 27 33
- PN 89-00002-00-084 Rev. B 29 35
- 30 PN 89-00002-00-084 Rev. B 36
- PN 89-00002-00-084 Rev. B 31 37
- 32 PN 89-00002-00-084 Rev. B 38
- Figure 21. Select Bead IDs 38
- PN 89-00002-00-084 Rev. B 33 39
- 34 PN 89-00002-00-084 Rev. B 40
- Maintenance 41
- Edit an Unused 42
- Edit a Used 43
- To edit a used template: 43
- Delete an Unused 44
- Delete a Used 45
- To delete a used template: 45
- Print Template 46
- Information 46
- PN 89-00002-00-084 Rev. B 41 47
- Technology 48
- To import a template: 49
- To export a template: 50
- About Developer 51
- Luminex Technical 53
- Support Website 53
- Handling 55
- Enumeration of xMAP 56
- Microsphere Suspensions 56
- PN 89-00002-00-084 Rev. B 51 57
- Protein Coupling Protocol 58
- Procedure 59
- Coupling 60
- Blocking, and 60
- PN 89-00002-00-084 Rev. B 55 61
- Equipment •Vortex 62
- PN 89-00002-00-084 Rev. B 57 63
- Enumerate the Coupled 64
- Microspheres 64
- LumAvidin Binding Protocol 65
- 60 PN 89-00002-00-084 Rev. B 66
- Version 1.7 67
- 62 PN 89-00002-00-084 Rev. B 68
- Customize Splash Screen 69
- 64 PN 89-00002-00-084 Rev. B 70
- PN 89-00002-00-084 Rev. B 65 71
- 66 PN 89-00002-00-084 Rev. B 72
- PN 89-00002-00-084 Rev. B 67 73
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