Luminex MasterPlex QT Tutorial Manual Manual de usuario

http://www.MiraiBio.com © 2007 Hitachi Software Engineering America, Ltd. All Rights Reserved. Tutorial

http://www.miraibio.com 10 MasterPlex QT You will then see the following dia

http://www.miraibio.com 11 MasterPlex QT Note for the IL-10 analyte, some

http://www.miraibio.com 12 MasterPlex QT lowest point on the standard curve,

http://www.miraibio.com 13 MasterPlex QT Marking and Grouping Wells We’ll o

http://www.miraibio.com 14 MasterPlex QT Navigate to C:\Program Files\Hitac

http://www.miraibio.com 15 MasterPlex QT We’ll mark A1 as background: Highl

http://www.miraibio.com 16 MasterPlex QT Now we will highlight B1 to H1, and

http://www.miraibio.com 17 MasterPlex QT The Standard wells will be grouped

http://www.miraibio.com 18 MasterPlex QT Now right-click on the wells, and s

http://www.miraibio.com 19 MasterPlex QT We can repeat this for the group C2

http://www.miraibio.com 2 MasterPlex QT Table of Contents Running Master

http://www.miraibio.com 20 MasterPlex QT Repeat this for groups E2, E3, E4,

http://www.miraibio.com 21 MasterPlex QT Designating the Standard/Known Conce

http://www.miraibio.com 22 MasterPlex QT Next, right-click on the highlight

http://www.miraibio.com 23 MasterPlex QT For analyte IL-5, the starting conc

http://www.miraibio.com 24 MasterPlex QT Now go back to the plate view, and

http://www.miraibio.com 25 MasterPlex QT Note if you click on a different an

http://www.miraibio.com 26 MasterPlex QT Select the Standard Curve Model No

http://www.miraibio.com 27 MasterPlex QT Select Five Parameter Logistics for

http://www.miraibio.com 28 MasterPlex QT Now click Exit in the Model Equatio

http://www.miraibio.com 29 MasterPlex QT If you wanted to save this informat

http://www.miraibio.com 3 MasterPlex QT Running MasterPlex QT using an exist

http://www.miraibio.com 30 MasterPlex QT Utilizing the Virtual Plate Feature

http://www.miraibio.com 31 MasterPlex QT To open a Virtual Plate, please cli

http://www.miraibio.com 32 MasterPlex QT This dialog screen allows a user

http://www.miraibio.com 33 MasterPlex QT Now, we are ready to merge our 2 s

http://www.miraibio.com 34 MasterPlex QT To dispense the wells, highlight we

http://www.miraibio.com 35 MasterPlex QT The Virtual Plate should now contai

http://www.miraibio.com 36 MasterPlex QT Now, let’s do the same thing with

http://www.miraibio.com 37 MasterPlex QT Highlight well E1 in the Virtual Pl

http://www.miraibio.com 38 MasterPlex QT Now, select and aspirate all the we

http://www.miraibio.com 39 MasterPlex QT The Virtual Plate now has all 10 b

http://www.miraibio.com 4 MasterPlex QT Select the Import a new plate opti

http://www.miraibio.com 40 MasterPlex QT Analyzing a single QuantigenePlex p

http://www.miraibio.com 41 MasterPlex QT 3. Designate well types: Select a w

http://www.miraibio.com 42 MasterPlex QT 4. Calculate results: Click the Cal

http://www.miraibio.com 43 MasterPlex QT a. Fold Change – This only will cal

http://www.miraibio.com 44 MasterPlex QT b. Fold Change – If the With normal

http://www.miraibio.com 45 MasterPlex QT Other Features to Explore Another

http://www.miraibio.com 46 MasterPlex QT You can right-click on the chart, a

http://www.miraibio.com 47 MasterPlex QT You can see Calculated, Expected,

http://www.miraibio.com 48 MasterPlex QT We’ll look at the Report per Unkno

http://www.miraibio.com 49 MasterPlex QT You will see the following report:

http://www.miraibio.com 5 MasterPlex QT Next, click the Open button shown ab

http://www.miraibio.com 50 MasterPlex QT Note that you can then go to the Re

http://www.miraibio.com 51 MasterPlex QT You can also contact MiraiBio with

http://www.miraibio.com 6 MasterPlex QT Click on the M5PlexReplicates te

http://www.miraibio.com 7 MasterPlex QT Also note the black borders that sho

http://www.miraibio.com 8 MasterPlex QT For this example, we will select the

http://www.miraibio.com 9 MasterPlex QT Click Exit to leave the Model Equa