Luminex xPONENT 3.1 Rev 2 (IVD) Manual de usuario descargar pdf (Pagina 105)

Term Definition

dot plot Graphical representation of microsphere analysis

classification data displayed by frequency and

intensity.

doublet discriminator (DD) A classification channel measuring side scatter;

used to filter for single bead events based on

size.

emission spectrum Wavelength range that an excited fluorochrome

emits when its electrons fall from a higher to a

lower energy state. Expressed in nanometers

(nm).

event Occurs when the signal processor determines

that a particle of any size has passed through the

lasers.

excitation spectrum Wavelength range that excites a molecule’s

electrons to a higher energy state. Expressed in

nanometers (nm).

fluorescence Light emission that occurs when the electrons of a

fluorochrome drop to a lower energy state.

fluorochrome A fluorescent molecule.

fluorophore See fluorochrome.

histogram The representation of frequency distribution for

analyte results. The DD channel results are often

displayed in a histogram format.

immunofluorescence A technique which uses a covalently linked

fluorochrome-antibody complex to detect or

quantify a particular antigen.

kit A group of standards and controls used to create

a protocol. They are used in the protocol’s

definition for the multi-analyte assays that will be

performed. Formerly called “product” in previous

releases of Luminex software.

Luminex xMAP microsphere set Luminex multi-analyte microspheres containing a

unique mixture of two distinctly colored

fluorochromes to distinguish them from other

multi-analyte microspheres.

laser Light Amplification by Stimulated Emission of

Radiation. This highly purified source of light is an

efficient way to excite fluorochrome electrons.

LIS Laboratory Information System; A system for

storing sample records and test requests.

lot A reagent with specific target values used for

analysis or calibration.

median fluorescence intensity (MFI) Used to denote how much fluorescence a given

particle population carries; expressed and

compared in linear numbers.

TABLE .

Continued

For In Vitro Diagnostic Use

Glossary

1 2 ... 100 101 102 103 104 105 106 107 108

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