Luminex MasterPlex CT Tutorial Manual Manual de usuario

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Tutorial for MasterPlex
®
GT v2
© 2006 Hitachi Software Engineering America, Ltd. All Rights Reserved.
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Pagina 1

Tutorial for MasterPlex® GT v2 © 2006 Hitachi Software Engineering America, Ltd. All Rights Reserved.

Pagina 2

http://www.miraibio.comPlex GT 10 To view the Allelic Frequencies, click on the Allele Frequency tab on the top. The count or frequency of each a

Pagina 3

http://www.miraibio.comPlex GT 11 Close Window Haplotype Frequency tab To view the Haplotype Frequency, simply click on the Haplotype Fr

Pagina 4 - Loci shown as tabs

http://www.miraibio.comPlex GT 12 Analyzing the Data with the Graph Window MasterPlex GT enables the user to view the data in the form of bar grap

Pagina 5

http://www.miraibio.comPlex GT 13 Toggle 2D and 3D Button Toggle MFI and RI values Show Dendrogram B

Pagina 6 - Selecting Background Samples

http://www.miraibio.comPlex GT 14 Heatmap Depth Bar Graph Tab

Pagina 7

http://www.miraibio.comPlex GT 15 MasterPlex GT offers two ways of analyzing and comparing a pair of samples or alleles in the Graph window: 1) Sa

Pagina 8

http://www.miraibio.comPlex GT 16 Allele by Allele Tab To perform analysis of two alleles, press the Allele by Allele Tab. Next, s

Pagina 9

http://www.miraibio.comPlex GT 17 MasterPlex GT is capable of displaying the pairwise comparisons for all loci based on correlation coefficients wi

Pagina 10

http://www.miraibio.comPlex GT 18 Homology Chart Tab To view a 3D graphical representation of the Homology Table, press the Homolo

Pagina 11

http://www.miraibio.comPlex GT 19 Printing Reports MasterPlex GT allows the user to print the following reports:  Sample Information including

Pagina 12

http://www.miraibio.comPlex GT 2 The MasterPlex GT software is designed for genotyping analysis of Single Nucleotide Polymorphisms (SNPs) and Human

Pagina 13

http://www.miraibio.comPlex GT 20 Open Save Print Close The Preview window enables the user to Open, Save, and Pri

Pagina 14

http://www.miraibio.comPlex GT 21 Congratulations! You have just finished analyzing the SNP data file utilizing MasterPlex GT v2. As you can see,

Pagina 15

http://www.miraibio.comPlex GT 22 You can contact MiraiBio with any questions you have at (510) 337-2000 (USA phone number) or by sending an email t

Pagina 16 - Allele by Allele Tab

http://www.miraibio.comPlex GT 3 Using the Typing Table Let’s start by opening a sample SNP data file. Press File and select Open Data File. Op

Pagina 17 - Show Homology Chart

http://www.miraibio.comPlex GT 4 View data by Locus Loci shown as tabs To view data by locus, press the By Locus button and the loci will appe

Pagina 18 - Homology Chart Tab

http://www.miraibio.comPlex GT 5 Gradient Background view Press the Gradient Background button to view the intensity of the MFI values. The dar

Pagina 19 - Report Manager Icon

http://www.miraibio.comPlex GT 6 Selecting Background Samples Wells A1 and D3 are the background samples for this particular data file. To set t

Pagina 20

http://www.miraibio.comPlex GT 7 Setting Up the Allele Call Parameters MasterPlex GT v2 allows the user to set the stringency of allele calling u

Pagina 21

http://www.miraibio.comPlex GT 8 The Parameter Setting window allows the user to insert the Allele Call parameters:  Minimum Events  Rela

Pagina 22

http://www.miraibio.comPlex GT 9 Utilizing the Allele Call Window Press the Bell icon to view the Allele Calling results Paint Background toggl

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