Luminex xPONENT 3.1 Manual de usuario descargar pdf (Pagina 38)

Luminex xPONENT 3.1 Software Manual

^ f) and the widely used Logic 5P regression fomula is calculated

as y = a + ((b-a)/(1 + ((x/c) ^ d )) ^ f).

NOTE: Luminex recommends Fit of All Standards as the most accurate

calculation of the curve fit.

Based on a range of quantitative, numerical results, a threshold range can be applied

to a quantitative analysis, for example, high, low, saturated, and expected.

• Allele Call - Sets analysis for an allele call. Analytes must put in groups of 2, 3, or 4

analytes.

• Min MFI Enabled - Select this box to enable a minimum MFI for the Allele Call

analysis. Type a value in this box to set the minimum MFI for analysis.

• Number of Standards - Click to type the number of standards for the protocol.

• Number of Controls - Click to type the number of controls for the protocol.

• Fit of all Standards - The standard curve will be determined by using each individual

standard replicate when calculating the standard curve. For example if one runs

duplicates of a 7 point standard curve, the software will calculate the standard curve by

using 14 points.

• Mean of Replicates - The standard curve will be determined by averaging the

individual standard replicates when calculating the standard curve. For example if one

runs duplicates of a 7-point standard curve, the software will calculate the standard

curve by using 7 averaged points.

• Analyze Results While Acquiring Samples - The software allows for real-time

viewing of the results as the instrument analyzes samples.

• Use External Analysis Program - Select this check-box to use a third-party program

to analyze the data. The Analysis Program list becomes active when this is selected.

• Analysis Program - Use this list to select which program to use for data analysis.

Cancel. Click to return to the main Batches tab.

Next:. Click to advance to the Analytes tab.

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Luminex xPONENT 3.1 Manual de usuario Pagina 38