Luminex xPONENT 4.0 SP1 Manual de usuario descargar pdf (Pagina 69)

Touring the Software

FIGURE 38. Settings Tab

This tab contains the following:

Name and Description boxes. Type a name and a description in the appropriate boxes.

Acquisition Settings. Use this section to set the following:

• Bead Type - Select either MicroPlex (standard) or MagPlex (magnetic) beads.

• Volume - This is the volume the instrument will aspirate into the system for analysis.

Type the desired sample volume in microliters. Use values from 10 to 200 µl for both

96-well and 384-well plates. To avoid air intake, add at least 25 µl to the sample well in

addition to the sample size. The default value is 50 µl.

• Timeout - Select Enabled to enable a timeout. Acquisition stops once the timeout is

reached. Type a value from 1 to 250 seconds.

• XY heater - Select Enabled to enable the XY heater. In the box, type the desired value

in degrees Celsius. The temperature range is 35 to 60°C in 0.5 increments.

WARNING: Acquiring data before the heater has reached the proper

temperature can compromise test results.

• Plate Name - Click the Plate Name list and select Current 96-well plate, Current

384-well plate, or a plate that you have previously saved.

• DD gating - Use this to set the minimum and maximum DD gating values. Use values

within the range of 0 to 32767. In the first box, type the minimum value. In the second

box, type the maximum value. The default minimum and maximum values are 7500

and 13500 respectively.

• Reporter Gain - Select Standard PMT from this list to decrease the reporter gain

level. The default option is Enhanced PMT. Refer to assay application instructions for

more detailed information.

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Luminex xPONENT 4.0 SP1 Manual de usuario Pagina 69