Luminex 200 User Manual with LDS 1.7 Software Manual de usuario Pagina 10
- Pagina / 154
- Tabla de contenidos
- SOLUCIÓN DE PROBLEMAS
- MARCADORES
Valorado. / 5. Basado en revisión del cliente
iv PN 89-00002-00-150 Rev. A
Luminex 200 User Manual for LDS Version 1.7 xMAP Technology
Bead Detail Irregularities . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 8-11
Error Messages. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 8-13
Printing Errors . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 8-14
Verification. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 8-14
Protocols 9-1
xMAP Microsphere Handling . . . . . . . . . . . . . . . . . . . . . . . . . . . . 9-1
Microsphere Dispersion. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 9-1
Probe Sonicator . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 9-2
Bath Sonicator . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 9-2
Enumeration of xMAP Microsphere Suspensions. . . . . . . . . . 9-3
xMAP Microsphere Separation Methods. . . . . . . . . . . . . . . . . 9-3
xMAP Microsphere Agitation During Assay . . . . . . . . . . . . . 9-3
xMAP Microsphere Stability and Storage. . . . . . . . . . . . . . . . 9-4
Two-Step Carbodiimide Coupling of Protein to xMAP
Carboxylated Microspheres . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 9-4
Introduction . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 9-4
Equipment . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 9-4
Materials . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 9-4
Preparation. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 9-5
Procedure . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 9-5
Technical Notes . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 9-6
One-Step Carbodiimide Coupling of
Oligonucleotides to xMAP Carboxylated Microspheres . . . . . . . . 9-7
Introduction . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 9-7
Equipment . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 9-7
Materials . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 9-7
Preparation. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 9-8
Procedure . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 9-8
Technical notes . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 9-9
Binding Biotin-conjugated Molecules to
LumAvidin-Modified xMAP Microspheres. . . . . . . . . . . . . . . . . . 9-9
Introduction . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 9-9
Equipment . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 9-9
Materials . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 9-10
Preparation. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 9-10
Procedure . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 9-10
Technical notes . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 9-11
Glossary A-1
Product Numbers B-1
Hardware . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . B-1
Software . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . B-2
Reagents . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . B-2
Training . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . B-3
- Luminex 200 1
- User Manual for 1
- LDS Version 1.7 1
- EULA PN: 89-30000-00-070 6
- Contents 7
- Installation 4-1 8
- System Startup 5-1 8
- Data Acquisition 6-1 8
- Maintenance and Cleaning 7-1 9
- Troubleshooting 8-1 9
- Protocols 9-1 10
- Glossary A-1 10
- Product Numbers B-1 10
- Index Index-1 11
- Introduction 13
- Fluorescence 16
- Compensation 16
- Repetitive Microsphere 16
- Measurements 16
- Technical 17
- Assistance 17
- Safety and 20
- Regulatory Symbols 20
- Mechanical 25
- Biological 25
- Blue Indicator Light 26
- System Overview 27
- Analyzer 29
- Specifications 29
- Luminex XYP 30
- Uninterruptible power 31
- Luminex System 32
- Overview 32
- Instrument 33
- Ventilation Filter 33
- See Figure 3-4 35
- Luminex XYP Heater 38
- Installation 39
- To set up the PC: 40
- Software Setup 41
- Adjusting the 42
- Sample Probe 42
- Note: To avoid adjusting the 43
- Installing Software 44
- System Startup 47
- Add New Classification 48
- Channel Calibration Lots 48
- Add New Reporter 50
- Calibrate the Luminex 51
- Preview or Print 53
- Calibration Report 53
- Figure 5-9. Print Dialog Box 54
- Figure 5-10. Calibration Log 54
- Analyzer menu 55
- Data Acquisition 59
- Figure 6-2. 100 Region MAP 60
- °C and 60°C (95°F 63
- Create a Session Using 64
- Default Settings 64
- Create a Session Using a 64
- Template 64
- Open and Add to an 66
- Existing Session 66
- Open an Existing Session 67
- Multiplex 68
- Main Control and Session 70
- To autoscale the Y axis: 75
- To set a gate: 76
- Bar Graph/Dot Plot 78
- Status Bar 82
- Run an Entire Plate of 83
- Interrupt a Run 85
- To print reports: 86
- Generic/Text Only 87
- Maintenance and Cleaning 89
- Clean Accessible 92
- Surfaces 92
- Replace Air Intake 93
- Warning: The syringe arm does 94
- Clean Luminex 95
- Analyzer Ventilation 95
- Change Sheath 95
- Quick-disconnect 96
- Adjust Sample 97
- Probe Vertical 97
- Decontaminating 99
- Analyzer for Return 99
- Shipment 99
- Troubleshooting 103
- Power Supply 104
- Problems 104
- Calibration 109
- Acquisition 111
- Bead Detail 113
- Irregularities 113
- Error Messages 115
- Printing Errors 116
- Verification 116
- Protocols 119
- Stability and Storage 122
- Equipment •Vortex 122
- Binding Biotin 127
- Molecules to 127
- LumAvidin-Modified 127
- Glossary 131
- Product Numbers 137
- Software 138
- Reagents 138
- Training 139
- Specifications 141
- Sheath Delivery 143
- System Setup 143
- Using the Sheath 145
- Delivery System 145
- Numerics 149
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