Luminex 200 User Manual with LDS 1.7 Software Manual de usuario Pagina 134
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Luminex 200 User Manual for LDS Version 1.7 xMAP Technology
xMAP microsphere set Luminex multi-analyte microspheres containing a unique mixture of
two distinctly-colored fluorochromes to distinguish them from other
multi-analyte microspheres.
laser Light Amplification by Stimulated Emission of Radiation (laser).
This highly purified source of light is an efficient way to excite
fluorochrome electrons.
LumAvidin
microspheres
An xMAP microsphere set that has been chemically modified so that
its surfaces are covered with avidin.
MAP Multiple Analyte Profile.
microparticle A solid substance with a diameter in the micrometer range. Often
used as a synonym for an xMAP microsphere.
microspheres Polystyrene spheres with a diameter in the micrometer range. Also
called beads.
multi-analyte Several assays or tests performed simultaneously in the same
reaction container.
multi-analyte panel A particular group of tests or assays performed simultaneously on
distinct xMAP microsphere sets.
particle In this application, used to describe a microsphere.
PE See Phycoerythrin.
photobleaching The process in which light absorption converts the fluorochromes
inside the beads to different fluorescent or nonfluorescent
compounds. Photobleaching prevents beads from being properly
classified.
PMT Photomultiplier tube, which measures the excitation emission.
Phycoerythrin (PE) A popular red fluorochrome commonly used as a reporter. Excitation
= 546 nm, Emission = 575 nm.
quenching Process by which a fluorochrome is affected by itself or another
fluorochrome in the same solution because of the close proximity of
the excitation and emission peaks.
region A designated area specific to a particular bead ID.
- Luminex 200 1
- User Manual for 1
- LDS Version 1.7 1
- EULA PN: 89-30000-00-070 6
- Contents 7
- Installation 4-1 8
- System Startup 5-1 8
- Data Acquisition 6-1 8
- Maintenance and Cleaning 7-1 9
- Troubleshooting 8-1 9
- Protocols 9-1 10
- Glossary A-1 10
- Product Numbers B-1 10
- Index Index-1 11
- Introduction 13
- Fluorescence 16
- Compensation 16
- Repetitive Microsphere 16
- Measurements 16
- Technical 17
- Assistance 17
- Safety and 20
- Regulatory Symbols 20
- Mechanical 25
- Biological 25
- Blue Indicator Light 26
- System Overview 27
- Analyzer 29
- Specifications 29
- Luminex XYP 30
- Uninterruptible power 31
- Luminex System 32
- Overview 32
- Instrument 33
- Ventilation Filter 33
- See Figure 3-4 35
- Luminex XYP Heater 38
- Installation 39
- To set up the PC: 40
- Software Setup 41
- Adjusting the 42
- Sample Probe 42
- Note: To avoid adjusting the 43
- Installing Software 44
- System Startup 47
- Add New Classification 48
- Channel Calibration Lots 48
- Add New Reporter 50
- Calibrate the Luminex 51
- Preview or Print 53
- Calibration Report 53
- Figure 5-9. Print Dialog Box 54
- Figure 5-10. Calibration Log 54
- Analyzer menu 55
- Data Acquisition 59
- Figure 6-2. 100 Region MAP 60
- °C and 60°C (95°F 63
- Create a Session Using 64
- Default Settings 64
- Create a Session Using a 64
- Template 64
- Open and Add to an 66
- Existing Session 66
- Open an Existing Session 67
- Multiplex 68
- Main Control and Session 70
- To autoscale the Y axis: 75
- To set a gate: 76
- Bar Graph/Dot Plot 78
- Status Bar 82
- Run an Entire Plate of 83
- Interrupt a Run 85
- To print reports: 86
- Generic/Text Only 87
- Maintenance and Cleaning 89
- Clean Accessible 92
- Surfaces 92
- Replace Air Intake 93
- Warning: The syringe arm does 94
- Clean Luminex 95
- Analyzer Ventilation 95
- Change Sheath 95
- Quick-disconnect 96
- Adjust Sample 97
- Probe Vertical 97
- Decontaminating 99
- Analyzer for Return 99
- Shipment 99
- Troubleshooting 103
- Power Supply 104
- Problems 104
- Calibration 109
- Acquisition 111
- Bead Detail 113
- Irregularities 113
- Error Messages 115
- Printing Errors 116
- Verification 116
- Protocols 119
- Stability and Storage 122
- Equipment •Vortex 122
- Binding Biotin 127
- Molecules to 127
- LumAvidin-Modified 127
- Glossary 131
- Product Numbers 137
- Software 138
- Reagents 138
- Training 139
- Specifications 141
- Sheath Delivery 143
- System Setup 143
- Using the Sheath 145
- Delivery System 145
- Numerics 149
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