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200 User Manual with LDS 1.7 Software
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vi
PN 89-00002-00-150 Rev. A
Luminex 200
User Manual for LDS Version 1.7
x
MAP Technology
1
2
...
7
8
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10
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12
13
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17
...
153
154
Luminex 200
1
User Manual for
1
LDS Version 1.7
1
EULA PN: 89-30000-00-070
6
Contents
7
Installation 4-1
8
System Startup 5-1
8
Data Acquisition 6-1
8
Maintenance and Cleaning 7-1
9
Troubleshooting 8-1
9
Protocols 9-1
10
Glossary A-1
10
Product Numbers B-1
10
Index Index-1
11
Introduction
13
Fluorescence
16
Compensation
16
Repetitive Microsphere
16
Measurements
16
Technical
17
Assistance
17
Safety and
20
Regulatory Symbols
20
Mechanical
25
Biological
25
Blue Indicator Light
26
System Overview
27
Analyzer
29
Specifications
29
Luminex XYP
30
Uninterruptible power
31
Luminex System
32
Overview
32
Instrument
33
Ventilation Filter
33
See Figure 3-4
35
Luminex XYP Heater
38
Installation
39
To set up the PC:
40
Software Setup
41
Adjusting the
42
Sample Probe
42
Note: To avoid adjusting the
43
Installing Software
44
System Startup
47
Add New Classification
48
Channel Calibration Lots
48
Add New Reporter
50
Calibrate the Luminex
51
Preview or Print
53
Calibration Report
53
Figure 5-9. Print Dialog Box
54
Figure 5-10. Calibration Log
54
Analyzer menu
55
Data Acquisition
59
Figure 6-2. 100 Region MAP
60
°C and 60°C (95°F
63
Create a Session Using
64
Default Settings
64
Create a Session Using a
64
Template
64
Open and Add to an
66
Existing Session
66
Open an Existing Session
67
Multiplex
68
Main Control and Session
70
To autoscale the Y axis:
75
To set a gate:
76
Bar Graph/Dot Plot
78
Status Bar
82
Run an Entire Plate of
83
Interrupt a Run
85
To print reports:
86
Generic/Text Only
87
Maintenance and Cleaning
89
Clean Accessible
92
Surfaces
92
Replace Air Intake
93
Warning: The syringe arm does
94
Clean Luminex
95
Analyzer Ventilation
95
Change Sheath
95
Quick-disconnect
96
Adjust Sample
97
Probe Vertical
97
Decontaminating
99
Analyzer for Return
99
Shipment
99
Troubleshooting
103
Power Supply
104
Problems
104
Calibration
109
Acquisition
111
Bead Detail
113
Irregularities
113
Error Messages
115
Printing Errors
116
Verification
116
Protocols
119
Stability and Storage
122
Equipment •Vortex
122
Binding Biotin
127
Molecules to
127
LumAvidin-Modified
127
Glossary
131
Product Numbers
137
Software
138
Reagents
138
Training
139
Specifications
141
Sheath Delivery
143
System Setup
143
Using the Sheath
145
Delivery System
145
Numerics
149
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