Luminex MAGPIX User Quick Guide Manual de usuario

MAGPIX® User QuickGuide 4.2

System InitializationSystem initialization prepares the system for data acquisition. On initial startup, the SystemInitialization should consist of:•

1. Load the CAL/VER CD (provided with the kit) on the computer.2. On the Home page, click System Initialization.3. Click Import Kit at the bottom righ

Defining the System Initialization RoutineNOTE: Luminex recommends daily performance verification and weeklycalibration of the MAGPIX system. You can

3. Under Maintenance Options, select Fluidics prep, performance verification from thedrop-down list. FIGURE 2.Setting System Initialization Routine4.

Creating a Quantitative Assay ProtocolThe protocol must contain multiple standards. The standards are assigned lot valueinformation for each test. A s

15. In the Analysis Settings dialog box, select the analysis method from the Method list,and the weighting in the Weight Type list. Click Apply to All

27. Click Next. The Plate Layout tab opens. • To add well commands, highlight the appropriate wells and mark them as unknown,standard, control, backgr

default Post Batch routine can be changed in the Batch Optionstab of the Admin page.Creating a KitTo create a kit:1. Open the Protocols page, then ope

Create a New Batch from an Existing ProtocolThis option creates a new batch using a selected protocol from the Installed Protocols list.The list conta

1. Open the Results page, then the Reports tab.2. In the Report drop-down list, select the category of report: batch, protocol, calibrationand verific

© Luminex Corporation, 2011. All rights reserved. No part of this publication may bereproduced, transmitted, transcribed, or translated into any langu

Shutting Down the AnalyzerRun the daily shutdown routine to prevent clogs and crystallization of salt in the sampleprobe. Clogs and crystallization of

MAGPIX® User Quick Guide 4.216

If you are not successful troubleshooting problems with MAGPIX, contact Luminex TechnicalSupport. See Technical Support. If necessary, attach a suppor

FIGURE 3.MAGPIX Off-Plate Reagent Block1 Reservoirs2 Strip WellsMAGPIX New System Startup ErrorsProblem Resolution1. Check the sample probe height for

Problem Resolution(1x) Clean with 0.1 N NaOH (reservoir C)(3x) Rinse (reservoir D)Run Calibration routine. Verify that reagent is freshly vortexed. Us

Problem ResolutionVER (strip well B)Verify that reagent is freshly vortexed. Use 6 drops of reagent.(1x) Rinse (reservoir D)Fluidics 1 (strip well C)V

MAGPIX Calibration and Performance VerificationTroubleshooting GuideTroubleshooting21

TABLE 1.Fittings to CheckFitting Location, Step, andFittingFluid CompartmentStep 3: Drive Fluid FittingFluid CompartmentStep 4: Waste ContainerFitting

You can access the Technical Support website using a user name and password athttps://oraweb.luminexcorp.com/OA_HTML/jtflogin.jsp.By Phone Inside the

8. Verify the location where you want to store the file. To change the location, click Browse,then navigate to the new folder and click OK.9. Click Sa

MAGPIX® User Quick Guide 4.2ii

Table of ContentsChapter 1 Starting the MAGPIX System for the First Time ...1Starting xPONENT ...

MAGPIX® User Quick Guide 4.2iv

Detailed instructions on how to install the MAGPIX System are provided in the PC InstallationInstructions (89-30000-00-263) and Installing MAGPIX (89-

• Blue light indicator turns on.• Fans turn on.• Syringe pump initializes.• The filter wheel initializes.• Software connects to the instrument.NOTE: I

1. On the Home page, click Probe and Heater under Daily Activities. The Probe &Heater tab opens.2. Use well D6 (this is the center of a standard 9

FIGURE 1.Sample Probe Height AdjustmentRevive After Storage RoutineNOTE: The Revive After Storage routine is necessary when the systemruns for the fir